Michael Bartlett, Torgny Fornstedt and Mark Dickman elaborate on the possible reasons why ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC) has risen to such prominence for ...

Evaluation of: Stephanowitz H, Lange S, Lang D et al. Improved two-dimensional reversed phase–reversed phase LC-MS/MS approach for identification of peptide–protein interactions. J. Proteome Res. 11(2 ...

This paper describes a unique Prep-rP-HPLC technique that uses a C-18/C-8 derivatized silica coated with a hydrophobic quaternary ammonium salt or quaternary phosphonium salt that acts as an ...

Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

Figure 1: Schematic representation of the continuous FFE apparatus coupled off-line to RP-HPLC. Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. Due to its high ...

When coupled with mass spectrometry, liquid chromatography forms a powerful technique with high sensitivity and selectivity. The technology is especially relevant to the specific detection and ...