Placing UV detectors far away from a chromatographic column can be problematic. The increased extra column volume can cause band broadening, reducing the efficiency of a separation. Also, experiments ...

Analysts interested in organic molecules like dissolved organic matter or pharmaceuticals should consider quantifying organic molecules with the High Performance Liquid Chromatography (HPLC) ...

The Pistoia Alliance’s Methods Database Project is addressing a longstanding challenge in analytical science by creating a standardized, vendor-independent format for the digital exchange of ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

In this article, the authors demonstrate that a normal-phase chromatographic method was stability-indicating for a water-sensitive prodrug. The stress conditions using aqueous and non-aqueous ...

This is the final article in this series discussing HPLC oligonucleotide separations. This article will discuss LC/MS analysis of failure sequences, phosphorothioate modified siRNAs, CpG methylated ...

Qualifying a UV spectrophotometer in the deep UV presents particular problems due to a lack of suitable reference materials and variation in the optical quality of spectrophotometer cells in this ...