PCR amplification was performed in a total volume of 10 μl, containing 1 μl cDNA, 1 μl 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U ExTaq polymerase (Takara Bio, Inc.), 500 nM each ...

High tumor mutation burden (TMB) in many cancer types is associated with the production of tumor-specific neoantigens, a favorable outcome and response to immune checkpoint blockade (ICB) therapy.

Tuberculosis (TB) remains one of the world's most serious public health threats, with approximately one-quarter of the global population infected with Mycobacterium tuberculosis. While most infections ...

The interrupted non-coding regions in pre-mRNAs, termed “introns,” are excised by “splicing” to generate mature coding mRNAs that are translated into proteins. As human pre-mRNA introns vary in length ...

Pre-mRNA splicing in a subset of human short introns is governed by a distinct mechanism involving a new splicing factor Protein-coding genes carry the blueprint for protein production. In higher ...