Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

What is now a standard protein laboratory technique began as an act of desperation. What is now a standard protein laboratory technique began as an act of desperation. In the late 1960s, two ...

Tumor necrosis factor ligand superfamily member 9 (4-1BBL), also known as 4-1BB ligand, CD137L, or TNFSF9, is a cytokine that binds to TNFRSF9. It serves as the high-affinity ligand for 4-1BB and ...

G protein-coupled receptors (GPCR) are a large and diverse class of plasma membrane receptors involved in transduction of extracellular signals. The range of GPCR ligands is broad and includes small ...

A research team led by Prof. Xu Pingyong from the Institute of Biophysics of the Chinese Academy of Sciences has developed an ...

Healthy cells are constantly breaking down proteins and building up new ones with the help of enzymes aptly named unfoldases, because they unravel proteins tagged for destruction or recycling. Now, a ...

In drug discovery, high hopes ride on degradation-based methods as a strategy to target proteins that are not easily subdued by classical inhibitors. And in basic research, too, degradation can ...