This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf thymus ...

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

An interview with Ramya Prabakaran & Melody Chang from GenScript, at SfN 2018, discussing the major challenges for researchers carrying out western blotting, and how GenScript's automated systems are ...

Western blotting can often be more trouble than expected. While SDS-PAGE and western blotting protocols are straightforward, there are many critical decision points that lead to success or failure. In ...